Sterile Processing Technician Practice Exam 2025 – Complete All-in-One Study Guide for Success!

Question: 1 / 400

Prolonged soaking of instruments can cause the formation of what?

Bioburdens

Pathogens

Pyrogens

Biofilms

Prolonged soaking of instruments can lead to the formation of biofilms. Biofilms are structured communities of microorganisms that adhere to surfaces, often encased in a protective matrix of extracellular polymeric substances. When instruments are soaked for an extended period, especially in the presence of organic materials or moisture, bacteria and other microorganisms can settle on the surfaces, multiply, and eventually form biofilms.

Biofilms are of particular concern in sterile processing as they can be difficult to remove and may protect bacteria from disinfection and sterilization processes, making it easier for infections to spread. This is why appropriate soaking times and proper cleaning procedures are critical in the preparation of surgical instruments to avoid the development of biofilms and ensure instruments are safe for use.

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